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TMEM97 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16973

Novus Biologicals, part of Bio-Techne
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NBP3-16973-100ul
NBP3-16973-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for TMEM97 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DLQAVLPRELYPVEFRNLLKWYAKEFKDPLLQEPPAWFK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TMEM97 Antibody

Western Blot: TMEM97 Antibody [NBP3-16973]

Western Blot: TMEM97 Antibody [NBP3-16973]

Western Blot: TMEM97 Antibody [NBP3-16973] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunocytochemistry/ Immunofluorescence: TMEM97 Antibody [NBP3-16973]

Immunocytochemistry/ Immunofluorescence: TMEM97 Antibody [NBP3-16973]

Immunocytochemistry/Immunofluorescence: TMEM97 Antibody [NBP3-16973] - Staining of human cell line CACO-2 shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP3-16973]

Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP3-16973]

Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP3-16973] - Staining of human salivary gland shows low expression as expected.

Applications for TMEM97 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TMEM97

TMEM97, or MAC30, is a conserved transmembrane protein that helps regulate cellular cholesterol levels through colocalization with NPC1. TMEM97 has also been linked to several cancers including breast, pancreatic, colorectal and ovarian cancers.

Long Name

Sigma intracellular receptor 2

Alternate Names

MAC30, S2R, Sigma-2 receptor, Sigma2 receptor

Gene Symbol

TMEM97

Additional TMEM97 Products

Product Documents for TMEM97 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TMEM97 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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