TMPRSS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20984

Novus Biologicals, part of Bio-Techne
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NBP1-20984
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TMPRSS2 Antibodies »

Product Specifications

Immunogen

This TMPRSS2 Antibody was developed against a peptide with sequence C-TDWIYRQMRADG corresponding to internal region according to NP_005647.2.

Epitope

C terminus

Reactivity Notes

Expected from sequence similarity: Mouse (82%)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

53.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TMPRSS2 Antibody

Western Blot: TMPRSS2 Antibody [NBP1-20984]

Western Blot: TMPRSS2 Antibody [NBP1-20984]

Western Blot: TMPRSS2 Antibody [NBP1-20984] - Western blot analysis of Human pancreas and kidney lysates (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TMPRSS2 Antibody [NBP1-20984]

Immunohistochemistry-Paraffin: TMPRSS2 Antibody [NBP1-20984]

Immunohistochemistry-Paraffin: TMPRSS2 Antibody [NBP1-20984] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunohistochemistry-Paraffin: TMPRSS2 Antibody [NBP1-20984]

Immunohistochemistry-Paraffin: TMPRSS2 Antibody [NBP1-20984]

Immunohistochemistry-Paraffin: TMPRSS2 Antibody [NBP1-20984] - Staining of paraffin embedded Human Kidney. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Applications for TMPRSS2 Antibody

Application
Recommended Usage

Immunohistochemistry

6 - 8 ug/mL

Immunohistochemistry-Paraffin

6 - 8 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 0.5 ug/mL
Application Notes
Western blot: Approx 55-60 kDa band observed in Human pancreas and kidney lysates (calculated MW of 53.9 kDa according to NP_005647.2). Use in ICC/IF reported in scientific publication (PMID: 32511360).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TMPRSS2

TMPRSS2, also called Epitheliasin in mice, is a 492 amino acid type II transmembrane serine protease located on human chromosome 21q22.3 that encodes at least 2 isoforms. This glycosylated serine protease (theoretical molecular weight 70kDa) is regulated by androgens and expressed on the plasma membrane of human bronchial epithelial cells, nasal goblet cells, small intestine epithelia, gastrointestinal tract, the stomach, kidneys and pancreas, with the greatest abundance found in the prostate gland. While the physiological function of TMPRSS2 remains unknown, the serine protease domain undergoes autocleavage and the 32 kDa domain is secreted enabling its interaction with the extracellular matrix. Fusions between TMPRSS2 and the ETS transcription factor genes ERG, ETV1, and ETV4 have been reported in prostate cancer with the TMPRSS2 ERG gene fusion resulting in ERG overexpression in 40-80% of these cases and often producing a more aggressive phenotype. Androgen signaling is disrupted in prostate cancers with the TMPRSS2 ERG fusion which contributes to the switch from androgen dependent to androgen independent prostate cancer (1,2).

TMPRSS2 has also been shown to play a critical role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 at the cell surface to facilitate viral entry. TMPRSS2 mediates viral entry in a similar mechanism for other coronaviruses such as SARS-CoV and MERS. The broad-spectrum serine protease inhibitor, Camostat, is a TMPRSS2 inhibitor demonstrated to protect mice with lethal SARS-CoV infections (3).

References

1. Clark, J., Cooper, C. (2009) ETS gene fusions in prostate cancer. Nat Rev Urol 6, 429-439. PMID: 19657377

2. Duffy, MJ. (2014) Chapter One - PSA in Screening for Prostate Cancer: More Good than Harm or More Harm than Good? Adv Clin Chem. 66:1-23. PMID: 25344984

3. Shen LW, Mao HJ, Wu YL, Tanaka Y, Zhang W. (2017) TMPRSS2: A potential target for treatment of influenza virus and coronavirus infections. Biochimie. 142:1-10

Long Name

Transmembrane protease, serine 2

Alternate Names

Epitheliasin, PP9284, PRSS10

Entrez Gene IDs

7113 (Human)

Gene Symbol

TMPRSS2

UniProt

NP_005647.2 (Human)

Additional TMPRSS2 Products

Product Documents for TMPRSS2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TMPRSS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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