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TOE1 Antibody (1D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00114034-M01

Novus Biologicals, part of Bio-Techne
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H00114034-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

TOE1 (AAH09364, 1 a.a. ~ 510 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAADSDDGAVSAPAASDGGVSKSTTSGEELVVQVPVVDVQSNNFKEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGLACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGSS

Specificity

TOE1 - target of EGR1, member 1 (nuclear)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TOE1 Antibody (1D8)

Western Blot: TOE1 Antibody (1D8) [H00114034-M01]

Western Blot: TOE1 Antibody (1D8) [H00114034-M01]

Western Blot: TOE1 Antibody (1D8) [H00114034-M01] - TOE1 monoclonal antibody (M01), clone 1D8 Analysis of TOE1 expression in Jurkat.
Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01]

Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01]

Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01] - Analysis of monoclonal antibody to TOE1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.3 ug/ml.
Western Blot: TOE1 Antibody (1D8) [H00114034-M01]

Western Blot: TOE1 Antibody (1D8) [H00114034-M01]

Western Blot: TOE1 Antibody (1D8) [H00114034-M01] - Analysis of TOE1 over-expressed 293 cell line, cotransfected with TOE1 Validated Chimera RNAi ( Cat # H00114034-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOE1 monoclonal antibody (M01), clone 1D8 (Cat # H00114034-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for TOE1 Antibody (1D8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA, RNAi validation, and immunohistochemistry (paraffin).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TOE1

TOE1 inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediatesthe inhibitory growth effect of EGR1

Alternate Names

FLJ13949, target of EGR1 protein 1, target of EGR1, member 1 (nuclear)

Entrez Gene IDs

114034 (Human)

Gene Symbol

TOE1

Additional TOE1 Products

Product Documents for TOE1 Antibody (1D8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TOE1 Antibody (1D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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