TOE1 Antibody (1D8)
Novus Biologicals, part of Bio-Techne | Catalog # H00114034-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1D8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TOE1 (AAH09364, 1 a.a. ~ 510 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAADSDDGAVSAPAASDGGVSKSTTSGEELVVQVPVVDVQSNNFKEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGLACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGSS
Specificity
TOE1 - target of EGR1, member 1 (nuclear)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TOE1 Antibody (1D8)
Western Blot: TOE1 Antibody (1D8) [H00114034-M01]
Western Blot: TOE1 Antibody (1D8) [H00114034-M01] - TOE1 monoclonal antibody (M01), clone 1D8 Analysis of TOE1 expression in Jurkat.Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01]
Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01] - Analysis of monoclonal antibody to TOE1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.3 ug/ml.Western Blot: TOE1 Antibody (1D8) [H00114034-M01]
Western Blot: TOE1 Antibody (1D8) [H00114034-M01] - Analysis of TOE1 over-expressed 293 cell line, cotransfected with TOE1 Validated Chimera RNAi ( Cat # H00114034-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOE1 monoclonal antibody (M01), clone 1D8 (Cat # H00114034-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for TOE1 Antibody (1D8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA, RNAi validation, and immunohistochemistry (paraffin).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TOE1
Alternate Names
FLJ13949, target of EGR1 protein 1, target of EGR1, member 1 (nuclear)
Entrez Gene IDs
114034 (Human)
Gene Symbol
TOE1
UniProt
Additional TOE1 Products
Product Documents for TOE1 Antibody (1D8)
Product Specific Notices for TOE1 Antibody (1D8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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