Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TOE1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 460 and 510 of human Target of EGR1, Member 1 using the numbering given in entry NP_079353.3 (GeneID 114034).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOE1 Antibody

Immunoprecipitation: TOE1 Antibody [NBP2-04022]

Immunoprecipitation: TOE1 Antibody [NBP2-04022]

Immunoprecipitation: TOE1 Antibody [NBP2-04022] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TOE1 antibody used for IP at 6 ug/mg lysate. TOE1 was also immunoprecipitated by rabbit anti-TOE1 antibody , which recognizes a downstream epitope. For blotting immunoprecipitated TOE1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: TOE1 Antibody [NBP2-04022]

Immunoprecipitation: TOE1 Antibody [NBP2-04022]

Immunoprecipitation: TOE1 Antibody [NBP2-04022] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TOE1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. TOE1 was also immunoprecipitated by rabbit anti-TOE1 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunoprecipitation: TOE1 Antibody [NBP2-04022]

Immunoprecipitation: TOE1 Antibody [NBP2-04022]

Immunoprecipitation: TOE1 Antibody [NBP2-04022] - Detection of human TOE1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TOE1 antibody NBP2-04022 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. TOE1 was also immunoprecipitated by another rabbit anti-TOE1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for TOE1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TOE1

TOE1 inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediatesthe inhibitory growth effect of EGR1

Alternate Names

FLJ13949, target of EGR1 protein 1, target of EGR1, member 1 (nuclear)

Entrez Gene IDs

NP_079353.3 (Human)

Gene Symbol

TOE1

Additional TOE1 Products

Product Documents for TOE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TOE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.