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TOE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49587

Novus Biologicals, part of Bio-Techne
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NBP2-49587

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGS

Reactivity Notes

Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOE1 Antibody

Western Blot: TOE1 Antibody [NBP2-49587]

Western Blot: TOE1 Antibody [NBP2-49587]

Western Blot: TOE1 Antibody [NBP2-49587] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: TOE1 Antibody [NBP2-49587]

Immunocytochemistry/ Immunofluorescence: TOE1 Antibody [NBP2-49587]

Immunocytochemistry/Immunofluorescence: TOE1 Antibody [NBP2-49587] - Staining of human cell line CACO-2 shows localization to nuclear bodies.
Immunohistochemistry-Paraffin: TOE1 Antibody [NBP2-49587]

Immunohistochemistry-Paraffin: TOE1 Antibody [NBP2-49587]

Immunohistochemistry-Paraffin: TOE1 Antibody [NBP2-49587] - Staining in human testis and liver tissues using anti-TOE1 antibody. Corresponding TOE1 RNA-seq data are presented for the same tissues.

Applications for TOE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TOE1

TOE1 inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediatesthe inhibitory growth effect of EGR1

Alternate Names

FLJ13949, target of EGR1 protein 1, target of EGR1, member 1 (nuclear)

Gene Symbol

TOE1

Additional TOE1 Products

Product Documents for TOE1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TOE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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