Tollip Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81597

Novus Biologicals, part of Bio-Techne
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NBP1-81597
NBP1-81597-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Tollip Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKA

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261). Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tollip Antibody

Immunohistochemistry-Paraffin: Tollip Antibody [NBP1-81597]

Immunohistochemistry-Paraffin: Tollip Antibody [NBP1-81597]

Immunohistochemistry-Paraffin: Tollip Antibody [NBP1-81597] - Staining of human cerebral cortex, epididymis, liver and testis using Anti-TOLLIP antibody NBP1-81597 (A) shows similar protein distribution across tissues to independent antibody NBP1-81596 (B).
Western Blot: Tollip Antibody [NBP1-81597]

Western Blot: Tollip Antibody [NBP1-81597]

Western Blot: Tollip Antibody [NBP1-81597] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: Tollip Antibody [NBP1-81597]

Immunocytochemistry/ Immunofluorescence: Tollip Antibody [NBP1-81597]

Immunocytochemistry/Immunofluorescence: Tollip Antibody [NBP1-81597] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
View 6 more images

Applications for Tollip Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tollip

TOLLIP is a ubiquitin-binding protein that interacts with several components of the Toll-like receptor (TLR; see MIM 603030) signaling cascade. It is an essential regulator of inflammatory signaling and is involved in trafficking of interleukin-1 receptor (IL1R; MIM 147810) and turnover of IL1R-associated kinase (IRAK, or IRAK1; MIM 300283) (summary by Lo et al., 2009 (PubMed 19444506)).(supplied by OMIM)

Long Name

Toll Interacting Protein

Alternate Names

IL-1RAcPIP

Gene Symbol

TOLLIP

Additional Tollip Products

Product Documents for Tollip Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Tollip Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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