TOP1MT Antibody (rTOP1MT/488) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14279
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rTOP1MT/488
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human TOP1MT protein (Uniprot: Q969P6 )
Localization
Cytoplasm (Mitochondria)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13850).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Applications for TOP1MT Antibody (rTOP1MT/488) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
0.5-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: TOP1MT
Alternate Names
mitochondrial, mitochondrial DNA topoisomerase I, topoisomerase (DNA) I, mitochondrial
Gene Symbol
TOP1MT
Additional TOP1MT Products
Product Documents for TOP1MT Antibody (rTOP1MT/488) - Azide and BSA Free
Product Specific Notices for TOP1MT Antibody (rTOP1MT/488) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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