TOP1MT Antibody (TOP1MT/488)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44360

Novus Biologicals, part of Bio-Techne
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NBP2-44360-0.02mg
NBP2-44360-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # TOP1MT/488

Concentration

0.2 mg/ml

View all TOP1MT Antibodies »

Product Summary for TOP1MT Antibody (TOP1MT/488)

Immunogen

Recombinant full-length human TOP1MT protein (Uniprot: Q969P6 )

Localization

Cytoplasmic (Mitochondria)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47793)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TOP1MT Antibody (TOP1MT/488)

Western Blot: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]

Western Blot: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]

Western Blot: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] - Analysis of human Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488).
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] - Human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Flow Cytometry: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]

Flow Cytometry: TOP1MT Antibody (TOP1MT/488) [NBP2-44360]

Flow Cytometry: TOP1MT Antibody (TOP1MT/488) [NBP2-44360] - Analysis of PFA-fixed MCF-7 cells using TOP1MT Antibody (TOP1MT/488).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
View 1 more images

Applications for TOP1MT Antibody (TOP1MT/488)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TOP1MT

The TOP1MT gene encodes a mitochondrial DNA topoisomerase, an enzyme that controls and alters the topologic states of DNAduring transcription. This enzyme catalyzes the transient breaking and rejoining of DNA which allows the strands topass through one another

Alternate Names

mitochondrial, mitochondrial DNA topoisomerase I, topoisomerase (DNA) I, mitochondrial

Gene Symbol

TOP1MT

UniProt

Q969P6 (Human)

Additional TOP1MT Products

Product Documents for TOP1MT Antibody (TOP1MT/488)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TOP1MT Antibody (TOP1MT/488)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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