TOP1MT Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89473
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQYRADWKSREMKTRQ
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TOP1MT Antibody
![Immunocytochemistry/ Immunofluorescence: TOP1MT Antibody [NBP1-89473]](https://resources.bio-techne.com/images/products/TOP1MT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89473-img0006.jpg "Immunocytochemistry/ Immunofluorescence: TOP1MT Antibody [NBP1-89473]")
Immunocytochemistry/ Immunofluorescence: TOP1MT Antibody [NBP1-89473]
Immunocytochemistry/Immunofluorescence: TOP1MT Antibody [NBP1-89473] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.![Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP1-89473]](https://resources.bio-techne.com/images/products/TOP1MT-Antibody-Immunohistochemistry-Paraffin-NBP1-89473-img0005.jpg "Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP1-89473]")
Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP1-89473]
Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP1-89473] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.Applications for TOP1MT Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TOP1MT
Alternate Names
mitochondrial, mitochondrial DNA topoisomerase I, topoisomerase (DNA) I, mitochondrial
Gene Symbol
TOP1MT
Additional TOP1MT Products
Product Documents for TOP1MT Antibody
Product Specific Notices for TOP1MT Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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