TOP1MT Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93237

Novus Biologicals, part of Bio-Techne
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NBP2-93237-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TOP1MT Antibodies »

Product Summary for TOP1MT Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1). RATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOP1MT Antibody - Azide and BSA Free

Western Blot: TOP1MT AntibodyAzide and BSA Free [NBP2-93237]

Western Blot: TOP1MT AntibodyAzide and BSA Free [NBP2-93237]

Western Blot: TOP1MT Antibody [NBP2-93237] - Analysis of extracts of A-549 cells, using TOP1MT at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time
Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]

Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]

Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP2-93237] - Immunohistochemistry of paraffin-embedded Rat lung using TOP1MT Rabbit pAb (NBP2-93237) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]

Immunohistochemistry-Paraffin: TOP1MT Antibody - Azide and BSA Free [NBP2-93237]

Immunohistochemistry-Paraffin: TOP1MT Antibody [NBP2-93237] - Immunohistochemistry of paraffin-embedded Human colon using TOP1MT Rabbit pAb (NBP2-93237) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for TOP1MT Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:200-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TOP1MT

The TOP1MT gene encodes a mitochondrial DNA topoisomerase, an enzyme that controls and alters the topologic states of DNAduring transcription. This enzyme catalyzes the transient breaking and rejoining of DNA which allows the strands topass through one another

Alternate Names

mitochondrial, mitochondrial DNA topoisomerase I, topoisomerase (DNA) I, mitochondrial

Gene Symbol

TOP1MT

Additional TOP1MT Products

Product Documents for TOP1MT Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TOP1MT Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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