TOP2B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89527

Novus Biologicals, part of Bio-Techne
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NBP1-89527
NBP1-89527-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat, Insect - Drosophila

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TOP2B Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOP2B Antibody

Western Blot: TOP2B Antibody [NBP1-89527]

Western Blot: TOP2B Antibody [NBP1-89527]

Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: TOP2B Antibody [NBP1-89527]

Immunocytochemistry/ Immunofluorescence: TOP2B Antibody [NBP1-89527]

Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP1-89527] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527]

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human pancreas shows weak to moderate nuclear positivity in islets of Langerhans and exocrine glandular cells.
View 6 more images

Applications for TOP2B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:100

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-89527 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TOP2B

Topo II Beta antibody plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Further, it is implicated in drug resistance of tumor cells.

Long Name

DNA Topoisomerase II beta

Alternate Names

antigen MLAA-44, DNA topoisomerase 2-beta, DNA topoisomerase II beta, DNA topoisomerase II, 180 kD, DNA topoisomerase II, beta isozyme, EC 5.99.1.3, top2beta, TOPIIB, topo II beta, topoisomerase (DNA) II beta (180kD), topoisomerase (DNA) II beta 180kDa, topoisomerase II beta, topoisomerase IIb, U937 associated antigen

Gene Symbol

TOP2B

Additional TOP2B Products

Product Documents for TOP2B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TOP2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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