TOR/mTOR [p Ser2481] Antibody (3H11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26405

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3H11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TOR Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human TOR/mTOR [p Ser2481] [UniProt P42345]

Modification

p Ser 2481

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOR/mTOR [p Ser2481] Antibody (3H11)

TOR/mTOR [p Ser2481] Antibody (3H11)

Western Blot: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] -

Western Blot: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Positive Western Blot detected in A549 whole cell lysate, 293 whole cell lysate (treated with Calyculin A or EGF).
All lanes: TOR/mTOR [p Ser2481] Antibody at 1.33ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 289 KDa
Observed band size: 289 KDa
TOR/mTOR [p Ser2481] Antibody (3H11)

Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] -

Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Staining of Hela cells with TOR/mTOR [p Ser2481] Antibody (3H11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for TOR/mTOR [p Ser2481] Antibody (3H11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TOR

The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase which helps to mediate many cellular processes. Specifically, mTOR is invloved in the cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex, making mTOR antibodies useful tools for a number of important research areas.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Gene Symbol

MTOR

Additional TOR Products

Product Documents for TOR/mTOR [p Ser2481] Antibody (3H11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TOR/mTOR [p Ser2481] Antibody (3H11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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