Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # F11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TOR Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is mTOR.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TOR/mTOR Antibody (F11)

Western Blot: TOR/mTOR Antibody (F11) [NBP2-00217]

Western Blot: TOR/mTOR Antibody (F11) [NBP2-00217]

Western Blot: mTOR Antibody (F11) [NBP2-00217] - Reduced cell lysates prepared from Jurkat cell line with 5 ug/mL of Anti-Human/Mouse mTOR Purified. Bands were visualized using Anti-Mouse IgG HRP.

Applications for TOR/mTOR Antibody (F11)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This F11 antibody has been tested by western blot analysis of reduced cell lysates prepared from Jurkat cells. This can be used at 1-5 ug/mL. This F11 antibody has also been reported for use in immunoprecipitation and immunohistology staining of paraffin embedded tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TOR

This F11 monoclonal antibody reacts with human, mouse, and rat mammalian target of rapamycin (mTOR). This 289-kDa serine/threonine kinase is activated via phosphorylation at Ser2448 by the PI3K/Akt signaling pathway in response to growth factors, energy, nutrients, and stress. mTOR is highly conserved amongst eukaryotes and constitutively expressed in immune (e.g. T, B, NK, dendritic cells, mast cells, and neutrophils) and non-immune tissues (e.g. heart). mTOR exists as a member of two distinct complexes, mTORC1 and mTORC2, which have differing sensitivities to rapamycin and phosphorylation targets. Rapamycin inhibits mTOR activity by binding FKBP12, which causes dissociation of mTORC1 and mTORC2. This molecule plays a critical role in cell metabolism, survival, dendritic cell maturation and activation, and protein synthesis. Deregulation of mTOR has been implicated in a variety of cancers. Studies have also suggested a role for mTOR in Foxp3 expression and regulatory T cell differentiation.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Gene Symbol

MTOR

Additional TOR Products

Product Documents for TOR/mTOR Antibody (F11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TOR/mTOR Antibody (F11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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