Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29728793).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOR/mTOR Antibody

Western Blot: TOR/mTOR Antibody [NB100-240]

Western Blot: TOR/mTOR Antibody [NB100-240]

Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TOR/mTOR Antibody [NB100-240]

Immunoprecipitation: TOR/mTOR Antibody [NB100-240]

Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for TOR/mTOR Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:100-1:2000
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (Suzuki H et al).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TOR

FKBP 12 rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 235 kDa protein (symbolized RAFT 1) with significant homology to the Saccharomyces cerevisiae protein TOR 1 and has been shown to associate with the immunophilin FKBP 12 in a rapamycin-dependent fashion. The FKBP 12 rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP 12 rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Gene Symbol

MTOR

Additional TOR Products

Product Documents for TOR/mTOR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TOR/mTOR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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