TOR/mTOR [p Ser2448] Antibody

Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607]

Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.

ELISA: TOR/mTOR [p Ser2448] Antibody [NB600-607] - ELISA Results of TOR/TOR/mTOR [p Ser2448] antibody tested against BSA-conjugated non-phospho [purple line] and phospho [blue line] forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The working dilution is 1:37,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer, Goat Anti-Rabbit HRP conjugated and TMB substrate.

TOR/mTOR [p Ser2448] Antibody

affinity purified anti-mTOR pS 2448 antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] -

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Analysis of mTOR phosphorylation during microglial activation(A) Whole-cell lysates were prepared from microglial cells incubated for 2 h as indicated. Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel. The same blots were subsequently probed for beta-actin, lower gel. (B) Quantitation of densitometry where p-mTOR values are reflected relative to those for beta-actin. Data are expressed as means±S.E.M. of n=1 replicate for each group, each assayed in triplicates. Representative of two different experiments. Data were analyzed by one-way ANOVA followed by the Bonferroni's post hoc test. ***P<0.001 versus Control, °P<0.05 versus C-CM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24689533), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] -

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Analysis of mammalian target of rapamycin (mTOR) phosphorylation during microglial activation. Whole-cell lysates were prepared from microglial cells activated by control-conditioned medium (C-CM) (A) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFN gamma (LI-CM) (B). Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel & were subsequently probed for beta-actin, lower gel. Quantitation of densitometry where p-mTOR values are reflected relative to those for beta-actin. Data are expressed as means ± standard error of the mean of n = 1 replicate for each group, each assayed in triplicate: representative of two different experiments. Data were analyzed by one-way analysis of variance followed by Bonferroni post hoc test. ***P <0.001 versus control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25051975), licensed under a CC-BY license. Not internally tested by Novus Biologicals.