TORC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2352

Novus Biologicals, part of Bio-Techne
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NB100-2352
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all TORC1 Antibodies »

Product Summary for TORC1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 600 and the C-terminus (residue 634) of human Transducer of Regulated cAMP Response element-binding protein 1 using the numbering given in entry NP_056136.1 (GeneID 23373 ).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TORC1 Antibody

Western Blot: TORC1 Antibody [NB100-2352]

Western Blot: TORC1 Antibody [NB100-2352]

Western Blot: TORC1 Antibody [NB100-2352] - Detection of human TORC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TORC1 antibody NB100-2352 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: TORC1 Antibody [NB100-2352]

Immunohistochemistry: TORC1 Antibody [NB100-2352]

Immunohistochemistry: TORC1 Antibody [NB100-2352] - Detection of human TORC1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TORC1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: TORC1 Antibody [NB100-2352]

Western Blot: TORC1 Antibody [NB100-2352]

Western Blot: TORC1 Antibody [NB100-2352] - Detection of Human and Mouse TORC1 on HeLa whole cell lysate using NB100-2352. TORC1 was also immunoprecipitated by another rabbit anti-TORC1 antibody and NB100-2351.
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Applications for TORC1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TORC1

MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1 does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11;19) (q21;p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.

Long Name

Transducer of Regulated CREB protein 1

Alternate Names

CRTC1, MECT1, WAMTP1

Entrez Gene IDs

23373 (Human); 382056 (Mouse)

Gene Symbol

CRTC1

UniProt

NP_056136.1 (Human)

Additional TORC1 Products

Product Documents for TORC1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TORC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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