TORC1 Antibody

Western Blot: TORC1 Antibody [NBP1-77940]

Western Blot: TORC1 Antibody [NBP1-77940] - Western blot using affinity purified TORC1 antibody shows detection of enCanineenous TORC1 (lower arrowhead) and TORC1-MAML2 fusion protein (top arrowhead) in cell lysates. Lane 1 contains lysate from cells expressing TORC1 only. Lane 2 contains lysate from cells transfected with fusion protein. Lane 3 contains lysate from cells strongly expressing TORC1 and weakly expressing the fusion protein. Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000.

ELISA: TORC1 Antibody [NBP1-77940]

ELISA: TORC1 Antibody [NBP1-77940] - ELISA Results of TORC1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The working dilution is 1: 19,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate stabilizer, Goat Anti-Rabbit HRP conjugated and TMB substrate.

TORC1 Antibody

Western blot using affinity purified anti-MECT1 antibody shows detection of endogenous MECT1 (lower arrowhead) and MECT1-MAML2 fusion protein (top arrowhead) in cell lysates. Lane 1 contains lysate from cells expressing MECT1 only. Lane 2 contains lysate from cells transfected with fusion protein. Lane 3 contains lysate from cells strongly expressing MECT1 and weakly expressing the fusion protein. Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.