TORC2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22356

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TORC2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human Torc2 protein (between residues 600-693) [UniProt Q53ET0]

Reactivity Notes

Predicted to react in Rat and Bovine based on sequence identity.

Localization

Cytoplasm and nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TORC2 Antibody - BSA Free

Western Blot: TORC2 AntibodyBSA Free [NBP2-22356]

Western Blot: TORC2 AntibodyBSA Free [NBP2-22356]

Western Blot: Torc2 Antibody [NBP2-22356] - Western blot analysis of Torc2 in A. Raji, B. Hek293 and C. Jurkat cell lysates.
Immunocytochemistry/ Immunofluorescence: TORC2 Antibody - BSA Free [NBP2-22356]

Immunocytochemistry/ Immunofluorescence: TORC2 Antibody - BSA Free [NBP2-22356]

Immunocytochemistry/Immunofluorescence: TORC2 Antibody [NBP2-22356] - Torc2 Antibody [NBP2-22356] - Torc2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: TORC2 Antibody - BSA Free [NBP2-22356]

Immunohistochemistry: TORC2 Antibody - BSA Free [NBP2-22356]

Immunohistochemistry: Torc2 Antibody [NBP2-22356] - IHC staining of Torc2 in mouse thymus.
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Applications for TORC2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Simple Western

1:100

Western Blot

1.0 - 2.0 ug/ml
Application Notes
This Torc2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 85 kDa. In ICC/IF cytoplasmic staining was observed in Hela cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TORC2

CREB-regulated transcription coactivator 2 (CRTC2), also known as transducer of regulated cAMP response element-binding protein 2, Torc2, or transducer of CREB protein 2, is a transcriptional coactivator for both CREB and the human T-cell leukemia virus type I (HTLV-1). In its dephosphorylated state, Torc2 becomes an activator in the SIK/TORC signaling pathway, and acts as a central regulator of cAMP dependent gluconeogenic gene expression in the LKB1/AMPK/TORC2 pathway. While Torc2 is normally found in the nucleus, it is translocated to the cytoplasm upon phosphorylation. Torc2 is abundantly expressed in the thymus but can also be found in the spleen, ovary, lung and muscle (where it acts as an inducer of mitochondrial biogenesis). Current research suggests that Torc2 responds to plasma membrane stress by facilitating changes in cellular lipid synthesis, thus altering the composition of the plasma membrane (PMID: 22504275). Other research indicates that Torc2 plays a role in regulating cancer cell migration, invasion, and metastasis (PMID: 21339740).

Long Name

Transducer of Regulated CREB protein 1

Alternate Names

CRTC2

Entrez Gene IDs

200186 (Human); 74343 (Mouse)

Gene Symbol

CRTC2

UniProt

Q3U182, Q53ET0 (Human)

Additional TORC2 Products

Product Documents for TORC2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TORC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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