TPX2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88312

Novus Biologicals, part of Bio-Techne
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NBP1-88312
NBP1-88312-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TPX2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPX2 Antibody

Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312]

Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312]

Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Analysis in human testis and pancreas tissues using NBP1-88312 antibody. Corresponding TPX2 RNA-seq data are presented for the same tissues.
Western Blot: TPX2 Antibody [NBP1-88312]

Western Blot: TPX2 Antibody [NBP1-88312]

Western Blot: TPX2 Antibody [NBP1-88312] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NBP1-88312]

Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NBP1-88312]

Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NBP1-88312] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
View 4 more images

Applications for TPX2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TPX2

TPX2 is a microtubule cross-linking protein involved in spindle formation. During mitosis, it is strictly associated with the mitotic spindle, whereas during phases S and G2 it is diffusely distributed throughout the nucleus.

Alternate Names

C20orf1HCTP4, C20orf2, chromosome 20 open reading frame 1, Differentially expressed in cancerous and non-cancerous lung cells 2, differentially expressed in lung cells, DIL2, DIL-2FLS353, HCA519, Hepatocellular carcinoma-associated antigen 519, P100, p100GD:C20orf1, preferentially expressed in colorectal cancer, Protein fls353, REPP86, restricted expression proliferation associated protein 100, Restricted expression proliferation-associated protein 100, targeting protein for Xklp2, TPX2, microtubule-associated protein homolog, TPX2, microtubule-associated, homolog (Xenopus laevis)

Gene Symbol

TPX2

Additional TPX2 Products

Product Documents for TPX2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TPX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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