Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # H43

Concentration

1 mg/ml

View all TR alpha/NR1A1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the full length Human Thyroid hormone receptor protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43)

Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]

Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]

Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of TR alpha/NR1A1/Thyroid Hormone Receptor alpha protein using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Load: 10 ug. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band(s): Higher molceular weight bands could be due to PTMs.
Immunocytochemistry/ Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]

Immunocytochemistry/ Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]

Immunocytochemistry/Immunofluorescence: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody. (D) Hoechst (E) Composite.
Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]

Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822]

Immunohistochemistry: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43) [NBP2-79822] - Immunohistochemistry analysis using Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody, Clone H43 (NBP2-79822). Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha Monoclonal Antibody (NBP2-79822) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 4X.
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Applications for TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43)

Application
Recommended Usage

Immunohistochemistry

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 °C.

Background: TR alpha/NR1A1

Thyroid Hormone Receptor alpha is encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Long Name

Thyroid Hormone Receptor alpha

Alternate Names

NR1A1, THRA, v-ErbA1

Gene Symbol

THRA

Additional TR alpha/NR1A1 Products

Product Documents for TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (H43)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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