Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4C7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TR2/NR2C1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-237 of human NR2C1 (NP_001027458) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TR2/NR2C1 Antibody (OTI4C7) - Azide and BSA Free

Western Blot: TR2/NR2C1 Antibody (OTI4C7)Azide and BSA Free [NBP2-74576]

Western Blot: TR2/NR2C1 Antibody (OTI4C7)Azide and BSA Free [NBP2-74576]

Western Blot: TR2/NR2C1 Antibody (OTI4C7) - Azide and BSA Free [NBP2-74576] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C1.
Immunohistochemistry: TR2/NR2C1 Antibody (OTI4C7) - Azide and BSA Free [NBP2-74576]

Immunohistochemistry: TR2/NR2C1 Antibody (OTI4C7) - Azide and BSA Free [NBP2-74576]

Immunohistochemistry: TR2/NR2C1 Antibody (OTI4C7) - Azide and BSA Free [NBP2-74576] - Analysis of Adenocarcinoma of Human breast tissue.

Applications for TR2/NR2C1 Antibody (OTI4C7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TR2/NR2C1

NR2C1 encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Long Name

Testicular Orphan Nuclear Receptor 2

Alternate Names

NR2C1

Gene Symbol

NR2C1

Additional TR2/NR2C1 Products

Product Documents for TR2/NR2C1 Antibody (OTI4C7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TR2/NR2C1 Antibody (OTI4C7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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