Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4B1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TR4/NR2C2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-241 of human NR2C2(NP_003289) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

67.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free

Western Blot: TR4/NR2C2 Antibody (OTI4B1)Azide and BSA Free [NBP2-74577]

Western Blot: TR4/NR2C2 Antibody (OTI4B1)Azide and BSA Free [NBP2-74577]

Western Blot: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2C2.
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577]

Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577]

Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577]

Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577]

Immunohistochemistry: TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free [NBP2-74577] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
View 18 more images

Applications for TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TR4/NR2C2

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Long Name

Testicular Orphan Nuclear Receptor 4

Alternate Names

NR2C2, TAK1

Gene Symbol

NR2C2

Additional TR4/NR2C2 Products

Product Documents for TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TR4/NR2C2 Antibody (OTI4B1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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