TR4/NR2C2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81658

Novus Biologicals, part of Bio-Techne
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NBP1-81658
NBP1-81658-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TR4/NR2C2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGARQTGLLDPGMLVNIQQPLIREDGTVLLATDSKAETSQGALGTLANVVTSLANLSESLNNGDTSEIQPEDQSASEITRAFDTLAKALNTTDSSSSPSLADGIDTSGGGSIHVISRDQSTPIIEVEGPLLSDTHVTFKLTMPSPMP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TR4/NR2C2 Antibody

Western Blot: TR4/NR2C2 Antibody [NBP1-81658]

Western Blot: TR4/NR2C2 Antibody [NBP1-81658]

Western Blot: TR4/NR2C2 Antibody [NBP1-81658] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: TR4/NR2C2 Antibody [NBP1-81658]

Immunocytochemistry/ Immunofluorescence: TR4/NR2C2 Antibody [NBP1-81658]

Immunocytochemistry/Immunofluorescence: TR4/NR2C2 Antibody [NBP1-81658] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Western Blot: TR4/NR2C2 Antibody [NBP1-81658]

Western Blot: TR4/NR2C2 Antibody [NBP1-81658]

Western Blot: TR4/NR2C2 Antibody [NBP1-81658] - Analysis in human cell line HeLa.
View 4 more images

Applications for TR4/NR2C2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TR4/NR2C2

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Long Name

Testicular Orphan Nuclear Receptor 4

Alternate Names

NR2C2, TAK1

Gene Symbol

NR2C2

Additional TR4/NR2C2 Products

Product Documents for TR4/NR2C2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TR4/NR2C2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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