TRAF-2 Antibody (9A5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26271

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9A5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRAF-2 Antibodies »

Product Summary for TRAF-2 Antibody (9A5)

Immunogen

A synthesized peptide derived from Human TRAF-2 [UniProt Q12933]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF-2 Antibody (9A5)

TRAF-2 Antibody (9A5)

Immunoprecipitation: TRAF-2 Antibody (9A5) [NBP3-26271] -

Immunoprecipitation: TRAF-2 Antibody (9A5) [NBP3-26271] - Immunoprecipitating TRAF-2 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26271 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26271 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
TRAF-2 Antibody (9A5)

Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] -

Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] - Image of TRAF-2 Antibody (9A5) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TRAF-2 Antibody (9A5)

Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] -

Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] - Image of TRAF-2 Antibody (9A5) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Applications for TRAF-2 Antibody (9A5)

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TRAF-2

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. TRAF2 is typically described as a 501 amino acid (aa) protein, GenBank no. NP_066961.2. However, various TRAF2 isoforms have been described. Therefore the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed.

Long Name

TNF Receptor-Associated Factor 2

Alternate Names

TRAF2, TRAP3

Gene Symbol

TRAF2

Additional TRAF-2 Products

Product Documents for TRAF-2 Antibody (9A5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TRAF-2 Antibody (9A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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