TRAF-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56177
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Zebrafish
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids 1-17 (MPGFDYKFLEKPKRRLL) of human TRAF4 was used as immunogen; GenBank no. gi|22027622|ref|NP_004286.2|.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TRAF-4 Antibody
Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177]
Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177] - Formalin-fixed, paraffin embedded, normal human mammary epithelial tissue using th antibody. A-C: Strong TRAF4 staining is seen in the luminal epithelial cells of the interlobular terminal ducts (acini). However, there is absence of TRAF4 immunostaining in the myoepithelial cells along the basement membrane. B and C: higher magnifications of A. Data courtesy of Krajewska et al.Immunoprecipitation: TRAF-4 Antibody [NB100-56177]
Immunoprecipitation: TRAF-4 Antibody [NB100-56177] - The NB100-56177 TRAF4 antibody specifically immunoprecipitates TRAF4. Top: TRAFs 2,3,4,5, and 6 were in vitro translated in the presence of 35S-methionine, and immunoprecipiated using the TRAF4 antibody. The TRAF4 antibody immunoprecipitated only TRAF4 and did not recognize TRAFs 2, 3, 5, or 6. A reticulocyte primed with an empty pcDNA plasmid was used as a negative control. Bottom: SDS-PAGE visualization of the in vitro translated TRAFs. Data courtesy of Krajewska et al.Applications for TRAF-4 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000-1:5000
Immunoprecipitation
1:1000-1:5000
Western Blot
1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.02% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRAF-4
Long Name
TNF Receptor-Associated Factor 4
Alternate Names
CART1, MLN62, TRAF4
Gene Symbol
TRAF4
Additional TRAF-4 Products
Product Documents for TRAF-4 Antibody
Product Specific Notices for TRAF-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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