TRAILR1/TNFRSF10A Antibody (32A1380) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56747

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Flow - CS, Flow Cytometry, Flow Cytometry Control, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 32A1380

Format

BSA Free

Concentration

1.0 mg/ml

View all TRAILR1/TNFRSF10A Antibodies »

Product Specifications

Immunogen

This monoclonal antibody was raised against a peptide corresponding to amino acid 2 to 21 of human DR4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TRAILR1/TNFRSF10A Antibody (32A1380) - BSA Free

Western Blot: TRAILR1/TNFRSF10A Antibody (32A1380)BSA Free [NB100-56747]

Western Blot: TRAILR1/TNFRSF10A Antibody (32A1380)BSA Free [NB100-56747]

Western Blot: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Analysis of DR4 in Daudi cell lysate using DR4 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: TRAILR1/TNFRSF10A Antibody (32A1380)BSA Free [NB100-56747]

Simple Western: TRAILR1/TNFRSF10A Antibody (32A1380)BSA Free [NB100-56747]

Simple Western: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Simple Western lane view shows a specific band for TRAIL R1 in 0.5 mg/ml of Daudi lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Applications for TRAILR1/TNFRSF10A Antibody (32A1380) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 18004565)

Flow (Intracellular)

reported in scientific literature (PMID 17541421)

Flow Cytometry

1:20-1:2000

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 26044191)

Simple Western

20 ug/ml

Western Blot

2-4 ug/ml
Application Notes
A 57 kDa band should be observed. This antibody is routinely tested by western blot analysis. For other applications, please refer to appropriate published references for more information.

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAILR1/TNFRSF10A

Cells undergo programmed cell death (Apoptosis) in response to various stimuli. Apoptosis is essential for morphogenesis, tissue homeostasis, and host defense Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kB activation in many tissues and cells.

Long Name

TRAIL Receptor 1

Alternate Names

CD261, DR4, TNFRSF10A, TRAIL R1

Gene Symbol

TNFRSF10A

UniProt

O00220 (Human)

Additional TRAILR1/TNFRSF10A Products

Product Documents for TRAILR1/TNFRSF10A Antibody (32A1380) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRAILR1/TNFRSF10A Antibody (32A1380) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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