TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31343

Clone DR-4-02 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # DR-4-02

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all TRAILR1/TNFRSF10A Antibodies »

Product Specifications

Immunogen

Fusion protein (Human). Fusion protein containing the extracellular part of DR4 and the constant part of the heavy chain of the human IgG1 (NM_003844.3).

Localization

Cell Membrane

Specificity

The mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein

Scientific Data Images for TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free [NBP2-31343]

Immunocytochemistry/ Immunofluorescence: TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free [NBP2-31343]

Immunocytochemistry/Immunofluorescence: TRAIL R1/TNFRSF10A Antibody (DR-4-02) - Azide Free [NBP2-31343] - Staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with TRAIL-R1 expression plasmid using anti-human TRAIL-R1 (DR-4-02).
Flow Cytometry: TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free [NBP2-31343]

Flow Cytometry: TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free [NBP2-31343]

Flow Cytometry: TRAIL R1/TNFRSF10A Antibody (DR-4-02) - Azide Free [NBP2-31343] - Using the PE direct conjugate Surface staining of CD261-transfected HEK-293 cells with anti-CD261/TRAIL-R1 (DR-4-02) PE.
TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free Flow Cytometry: TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free [NBP2-31343]

Flow Cytometry: TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free [NBP2-31343]

Analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines.Cells were stained with purified anti-TRAIL-R1 purified antibodies (low endotoxin) followed by Goat anti-mouse IgG PE.

Applications for TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

2-4 ug/ml
Application Notes
Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3 ug/ml in cultivation medium Final concentration of TRAIL: 20-200 ng/ml It is recommended to add the antibody 15 min before addition of TRAIL. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 0.2 um filter sterilized

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRAILR1/TNFRSF10A

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL, which is a new member in the TNF family. DR4 is also called TRAIL receptor 1(TRAIL R1). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF kB activation in many tissues and cells.

Long Name

TRAIL Receptor 1

Alternate Names

CD261, DR4, TNFRSF10A, TRAIL R1

Gene Symbol

TNFRSF10A

Additional TRAILR1/TNFRSF10A Products

Product Documents for TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRAILR1/TNFRSF10A Antibody (DR-4-02) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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