Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 12A380

Concentration

0.5 mg/ml

View all TRANCE/TNFSF11/RANK L Antibodies »

Product Summary for TRANCE/TNFSF11/RANK L Antibody (12A380) [PE]

Immunogen

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.

Reactivity Notes

Cross reacts with Human and Mouse.

Localization

Flow Cytometry (Surface): please see Yoneda et al. 2004 for details. Immunohistochemistry-Frozen: please see Yoneda et al. 2004 for details.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TRANCE/TNFSF11/RANK L Antibody (12A380) [PE]

Flow (Cell Surface): TRANCE/TNFSF11/RANK L Antibody (12A380) [PE] [NBP2-27402PE] - Analysis in RAW cells using 2 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody.

Applications for TRANCE/TNFSF11/RANK L Antibody (12A380) [PE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Cell Surface)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TRANCE/TNFSF11/RANK L

TRANCE/OPGL/RANkL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANkL {receptor activator of NF-kB (RANk) ligand} has been implicated in interactions between T cells and dendritic cells (4-5). RANk ligand (RANkL/TRANCE) binds to RANk on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (4). TRANCE/RANkL is also important in T- and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (1,2). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for TRANCE/TNFSF11/RANK L Antibody (12A380) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody (12A380) [PE]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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