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Transcobalamin II Antibody (2F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006948-M01

Novus Biologicals, part of Bio-Techne
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H00006948-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

TCN2 (AAH11239, 1 a.a. ~ 400 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKSGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANWHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW

Specificity

TCN2 - transcobalamin II; macrocytic anemia (2F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Transcobalamin II Antibody (2F4)

Western Blot: Transcobalamin II Antibody (2F4) [H00006948-M01]

Western Blot: Transcobalamin II Antibody (2F4) [H00006948-M01]

Western Blot: Transcobalamin II Antibody (2F4) [H00006948-M01] - Analysis of TCN2 expression in transfected 293T cell line by TCN2 monoclonal antibody (M01), clone 2F4.Lane 1: TCN2 transfected lysate (Predicted MW: 47.5 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Transcobalamin II Antibody (2F4) [H00006948-M01] - Detection limit for recombinant GST tagged TCN2 is 1 ng/ml as a capture antibody.

Applications for Transcobalamin II Antibody (2F4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Transcobalamin II

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. [provided by RefSeq]

Alternate Names

TC-2, TCII, TCN2, Vitamin B12-binding Protein 2

Entrez Gene IDs

6948 (Human)

Gene Symbol

TCN2

Additional Transcobalamin II Products

Product Documents for Transcobalamin II Antibody (2F4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Transcobalamin II Antibody (2F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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