Transcription factor E3 Antibody (CL12822)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18540

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-18540-100ul
NBP3-18540-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL12822

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Transcription factor E3 Antibodies »

Product Summary for Transcription factor E3 Antibody (CL12822)

Immunogen

This antibody was developed against a recombinant protein of P19532. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Transcription factor E3 Antibody (CL12822)

Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Analysis in human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunocytochemistry/ Immunofluorescence: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunocytochemistry/Immunofluorescence: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of U2-OS cells using the Anti-TFE3 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human liver shows no positivity in hepatocytes as expected.
View 4 more images

Applications for Transcription factor E3 Antibody (CL12822)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 -1:2500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Transcription factor E3

TFE3, a member of the helix-loop-helix family of transcription factors, binds to the mu-E3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types (Henthorn et al., 1991 [PubMed 1685140]).[supplied by OMIM]

Alternate Names

BHLHE33, bHLHe33transcription factor E3, Class E basic helix-loop-helix protein 33, RCCP2, TFEA, transcription factor binding to IGHM enhancer 3, transcription factor E family, member A, Transcription factor for IgH enhancer

Gene Symbol

TFE3

Additional Transcription factor E3 Products

Product Documents for Transcription factor E3 Antibody (CL12822)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Transcription factor E3 Antibody (CL12822)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.