Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoassay, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Transferrin Antibodies »

Product Summary for Transferrin Antibody (8B9)

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Transferrin purified from pooled human plasma.

Localization

Secreted

Specificity

Human transferrin. Complex between transferrin and soluble transferrin receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Transferrin Antibody (8B9)

Immunoassay: Transferrin Antibody (8B9) [NB110-7904]

Immunoassay: Transferrin Antibody (8B9) [NB110-7904]

Immunoassay: Transferrin Antibody (8B9) [NB110-7904] - Titration curve of sTfR-Tf complex contained in normal human serum using sTfR-Tf assay. Capture: MAb 23D10; 200 ng/well Detection: MAb 8B9 or 11D3 conjugated with HRP Room temperature

Applications for Transferrin Antibody (8B9)

Application
Recommended Usage

ELISA

1:100-1:2000
Application Notes
NB110-8468 can be used for detection of a complex between transferrin and transferrin receptor in sandwich immunoassay using anti-transferrin NB110-7904 and NB120-10208 as detection antibody. Recommended pairs for sandwich immunoassay of transferrin-transferrin receptor complex (capture - detection): NB110-8468 (Capture) -NB110-7904 (Detection)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Transferrin

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Entrez Gene IDs

7018 (Human)

Gene Symbol

TF

Additional Transferrin Products

Product Documents for Transferrin Antibody (8B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Transferrin Antibody (8B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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