Transferrin Antibody (HTF-14) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-418

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine (Negative), Canine (Negative), Equine (Negative), Rabbit, Sheep (Negative)

Cited:

Human

Applications

Validated:

Block/Neutralize, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Radioimmunoassay, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HTF-14

Format

BSA Free

Concentration

1.0 mg/ml

View all Transferrin Antibodies »

Product Specifications

Immunogen

Purified porcine transferrin.

Localization

Secreted

Specificity

The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Transferrin Antibody (HTF-14) - BSA Free

Western Blot: Transferrin Antibody (HTF-14)BSA Free [NB500-418]

Western Blot: Transferrin Antibody (HTF-14)BSA Free [NB500-418]

Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ug/well (red. con.) M. Low Range marker 4. hTransferrin; 1 ug/well (non-red. con.) 5. hTransferrin; 3 ug/well (non-red. con.) 6. hTransferrin; 5 ug/well (non-red. con.)
Immunohistochemistry-Paraffin: Transferrin Antibody (HTF-14) - BSA Free [NB500-418]

Immunohistochemistry-Paraffin: Transferrin Antibody (HTF-14) - BSA Free [NB500-418]

Immunohistochemistry-Paraffin: Transferrin Antibody (HTF-14) [NB500-418] - Staining of human placenta (paraffin sections) using anti-transferrin (HTF-14).

Applications for Transferrin Antibody (HTF-14) - BSA Free

Application
Recommended Usage

ELISA

1:2000RadioImmunoassay 1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Radioimmunoassay

1:100-1:2000

Western Blot

1-2 ug/ml
Application Notes
Western Blot: use non-reducing conditions. The antibody HTF-14 blocks binding of transferrin to the receptor. Purity is > 95% (by SDS-PAGE).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB500-418 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Transferrin

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Entrez Gene IDs

7018 (Human)

Gene Symbol

TF

Additional Transferrin Products

Product Documents for Transferrin Antibody (HTF-14) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Transferrin Antibody (HTF-14) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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