Transglutaminase 2/TGM2 Antibody (SPM358)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44595
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey, Rabbit
Cited:
Rat
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM358
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Transglutaminase 2/TGM2 protein (Uniprot: P21980)
Localization
Cytoplasmic and cell surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47927)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Transglutaminase 2/TGM2 Antibody (SPM358)
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (SPM358) [NBP2-44595]
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (SPM358) [NBP2-44595] - Formalin-fixed, paraffin-embedded human Placenta stained with Transglutaminase 2/TGM2 Antibody (SPM592).Applications for Transglutaminase 2/TGM2 Antibody (SPM358)
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Transglutaminase 2/TGM2
Additional Transglutaminase 2/TGM2 Products
Product Documents for Transglutaminase 2/TGM2 Antibody (SPM358)
Product Specific Notices for Transglutaminase 2/TGM2 Antibody (SPM358)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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