TRAP1 Antibody (OTI1H8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74592

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1H8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TRAP1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TRAP1(NP_057376) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

79.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRAP1 Antibody (OTI1H8) - Azide and BSA Free

Western Blot: TRAP1 Antibody (OTI1H8)Azide and BSA Free [NBP2-74592]

Western Blot: TRAP1 Antibody (OTI1H8)Azide and BSA Free [NBP2-74592]

Western Blot: TRAP1 Antibody (OTI1H8) - Azide and BSA Free [NBP2-74592] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP1.
Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (OTI1H8) - Azide and BSA Free [NBP2-74592]

Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (OTI1H8) - Azide and BSA Free [NBP2-74592]

Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (OTI1H8) - Azide and BSA Free [NBP2-74592] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP1.
Immunohistochemistry: TRAP1 Antibody (OTI1H8) - Azide and BSA Free [NBP2-74592]

Immunohistochemistry: TRAP1 Antibody (OTI1H8) - Azide and BSA Free [NBP2-74592]

Immunohistochemistry: TRAP1 Antibody (OTI1H8) - Azide and BSA Free [NBP2-74592] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.
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Applications for TRAP1 Antibody (OTI1H8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRAP1

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is TRAP1. Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein. Immunofluorescence staining of TRAP1 in PC-3-M cells with this antibody produces a pattern consistent with mitochondrial staining. Immunoprecipitation of TRAP1 using this antibody fails to co-precipitate p23, Hop, or CyP40 suggesting TRAP1®s inability to associate with these co-chaperones.

Alternate Names

HSP 75, HSP75TNFR-associated protein 1, HSP90Lheat shock protein 75 kDa, mitochondrial, TNF receptor-associated protein 1, TRAP-1, tumor necrosis factor type 1 receptor associated protein, Tumor necrosis factor type 1 receptor-associated protein

Gene Symbol

TRAP1

Additional TRAP1 Products

Product Documents for TRAP1 Antibody (OTI1H8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRAP1 Antibody (OTI1H8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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