TRAP1 Antibody (TRAP1-6)

Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555]

Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Immunolocalization of TRAP1 in PC-3-M cells.

Immunohistochemistry: TRAP1 Antibody (TRAP1-6) [NB300-555] -

Immunohistochemistry: TRAP1 Antibody (TRAP1-6) [NB300-555] - Representative images of IHF staining of EP2 or EP4 (green) and TRAP1 (NB300-555) (red) in subchondral bone 2 weeks post-sham or ACLT surgery (left) and quantitative analysis (right). The white arrows indicate TRAP-positive osteoclasts expressing EP2 or EP4. Scale bars, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562/) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] -

Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - PGE2 regulates migration & osteoclast differentiation through the G alphas/PI3K/AKT/MAPK signaling pathways downstream of EP4. PGE2 regulates migration & osteoclast differentiation independent of beta-arrestin1 (a) & beta-arrestin2 (b) but via G alphas (c). BMMs from the WT & littermate beta-arrestin1- & beta-arrestin2-knockout mice were used to generate osteoclasts by stimulation with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL & incubation with 100 nmol·L−1 PGE2. For G alphas experiments, osteoclasts were generated from the GMMs of the G alphasfl/fl controls & G alphasfl/fl; LysM-cre (G alphasLysM) mice. A differentiation assay (TRAP staining) (left) & the corresponding quantitative analysis (right). Scale bars, 50 μm. Error bars are the mean ± s.d. n = 3. Two-way ANOVA followed by Tukey’s t tests. Scale bars, 50 μm. d cAMP production was measured via ELISAs for osteoclasts derived from the Ep4fl/fl & Ep4LysM mice. BMMs from the two mouse strains were isolated & stimulated with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL to differentiate into osteoclasts & incubated with 100 nmol·L−1 PGE2 for 30 min prior to cAMP measurements. Error bars are the mean ± s.d. ***P < 0.001 by unpaired two-tailed Student’s t test. The experiment was performed with three biological replicates. e Representative images of the indicated protein expression by western blotting for osteoclasts generated using BMMs of Ep4fl/fl & Ep4LysM mice. The cells were treated either with osteoclastogenic media (10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL) alone, with PGE2 (100 nmol·L−1), or PGE2 with IBMX (1 mmol·L−1) for 3 h. The experiments were performed with three biological replicates Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35260562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.