TRAP220/MED1 Antibody (1G3)

Novus Biologicals, part of Bio-Techne | Catalog # H00005469-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRAP220 Antibodies »

Product Specifications

Immunogen

PPARBP (NP_004765, 1391 a.a. ~ 1490 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLP

Specificity

PPARBP (1G3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TRAP220/MED1 Antibody (1G3)

ELISA: TRAP220/MED1 Antibody (1G3) [H00005469-M02]

ELISA: TRAP220/MED1 Antibody (1G3) [H00005469-M02]

ELISA: TRAP220/MED1 Antibody (1G3) [H00005469-M02] - Detection limit for recombinant GST tagged PPARBP is approximately 0.1ng/ml as a capture antibody.

Applications for TRAP220/MED1 Antibody (1G3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRAP220

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize.

Long Name

Thyroid Hormone Receptor-associated Protein Complex Component 220 kDa

Alternate Names

DRIP230, MBD4, MED1, PPARBP, PPARGBP, TRIP2

Entrez Gene IDs

5469 (Human)

Gene Symbol

MED1

OMIM

604311 (Human)

UniProt

NP_004765 (Human)

Additional TRAP220 Products

Product Documents for TRAP220/MED1 Antibody (1G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRAP220/MED1 Antibody (1G3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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