TRBP Antibody (PCRP-TARBP2-1E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07335

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-TARBP2-1E5

Concentration

0.2 mg/ml

View all TRBP Antibodies »

Product Summary for TRBP Antibody (PCRP-TARBP2-1E5)

Immunogen

Recombinant full-length human TRBP protein (Uniprot: Q15633)

Localization

Cytoplasm, Cytoplasm > perinuclear region. Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08235)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TRBP Antibody (PCRP-TARBP2-1E5)

Immunocytochemistry/ Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335]

Immunocytochemistry/ Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335]

Immunocytochemistry/Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Immunofluorescence Analysis of PFA-fixed HeLa cells using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335]

Flow Cytometry: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335]

Flow Cytometry: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335]

Protein Array: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335]

Protein Array: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5).

Applications for TRBP Antibody (PCRP-TARBP2-1E5)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TRBP

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Alternate Names

LOQS, RISC-loading complex subunit TARBP2, TAR (HIV) RNA binding protein 2, TAR (HIV) RNA-binding protein 2, TAR (HIV) RNA-binding protein TRBP1, TAR (HIV-1) RNA binding protein 2, TAR RNA binding protein 2, TAR RNA-binding protein 2, trans-activation responsive RNA-binding protein, Trans-activation-responsive RNA-binding protein, TRBP, TRBP1, TRBP2

Gene Symbol

TARBP2

UniProt

Q15633 (Human)

Additional TRBP Products

Product Documents for TRBP Antibody (PCRP-TARBP2-1E5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TRBP Antibody (PCRP-TARBP2-1E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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