Funcional: TREM-1 mediates the septic shock pathway, and blocking TREM-1 has been reported to reduce inflammation and increase survival in certain models of bacterial infection. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Endotoxin Level <0.01EU/ug. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TREM-1

Monocyte/macrophage- and neutrophil-mediated inflammatory responses can be stimulated through a variety of receptors, including G protein-linked 7-transmembrane receptors (e.g., FPR1; MIM 136537), Fc receptors (see MIM 146790), CD14 (MIM 158120) and Toll-like receptors (e.g., TLR4; MIM 603030), and cytokine receptors (e.g., IFNGR1; MIM 107470). Engagement of these receptors can also prime myeloid cells to respond to other stimuli. Myeloid cells express receptors belonging to the Ig superfamily, such as TREM1, or to the C-type lectin superfamily. Depending on their transmembrane and cytoplasmic sequence structure, these receptors have either activating (e.g., KIR2DS1; MIM 604952) or inhibitory functions (e.g., KIR2DL1; MIM 604936).[supplied by OMIM] Mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes. TREM-1 is involved in the microbial inflammatory response. It acts in conjunction with the adaptor protein DAP12 to activate neutrophils and monocytes, leading to the secretion of pro-inflammatory mediators and enhancement of the inflammatory response. Microbial infection also induces the release of soluble form of TREM-1 from monocytes, which is reported to act as an inhibitor of TREM-1.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

58217 (Mouse)

Gene Symbol

TREM1

UniProt

Q9JKE2

Additional TREM-1 Products

Product Documents for TREM1 Antibody (L5-B8.2A12.3A12)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for TREM1 Antibody (L5-B8.2A12.3A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

TREM1 Antibody (L5-B8.2A12.3A12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40907

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all TREM-1 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for TREM1 Antibody (L5-B8.2A12.3A12)

Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Applications for TREM1 Antibody (L5-B8.2A12.3A12)

Flow Cytometry

Immunohistochemistry

Immunohistochemistry-Frozen

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: TREM-1

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional TREM-1 Products

Product Documents for TREM1 Antibody (L5-B8.2A12.3A12)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for TREM1 Antibody (L5-B8.2A12.3A12)