Key Product Details

Species Reactivity

Validated:

Mouse

Applications

CyTOF-ready, Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # L5-B8.2A12.3A12

Concentration

1.0 mg/ml

View all TREM-1 Antibodies »

Product Summary for TREM1 Antibody (L5-B8.2A12.3A12)

Immunogen

Mouse TREM-1 Fc fusion protein

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for TREM1 Antibody (L5-B8.2A12.3A12)

Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor 647
Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:RPE.
Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Flow Cytometry: TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free [NBP1-40907]

Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor 488

Applications for TREM1 Antibody (L5-B8.2A12.3A12)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Funcional: TREM-1 mediates the septic shock pathway, and blocking TREM-1 has been reported to reduce inflammation and increase survival in certain models of bacterial infection. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Endotoxin Level <0.01EU/ug. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TREM-1

Monocyte/macrophage- and neutrophil-mediated inflammatory responses can be stimulated through a variety of receptors, including G protein-linked 7-transmembrane receptors (e.g., FPR1; MIM 136537), Fc receptors (see MIM 146790), CD14 (MIM 158120) and Toll-like receptors (e.g., TLR4; MIM 603030), and cytokine receptors (e.g., IFNGR1; MIM 107470). Engagement of these receptors can also prime myeloid cells to respond to other stimuli. Myeloid cells express receptors belonging to the Ig superfamily, such as TREM1, or to the C-type lectin superfamily. Depending on their transmembrane and cytoplasmic sequence structure, these receptors have either activating (e.g., KIR2DS1; MIM 604952) or inhibitory functions (e.g., KIR2DL1; MIM 604936).[supplied by OMIM] Mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes. TREM-1 is involved in the microbial inflammatory response. It acts in conjunction with the adaptor protein DAP12 to activate neutrophils and monocytes, leading to the secretion of pro-inflammatory mediators and enhancement of the inflammatory response. Microbial infection also induces the release of soluble form of TREM-1 from monocytes, which is reported to act as an inhibitor of TREM-1.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

58217 (Mouse)

Gene Symbol

TREM1

UniProt

Q9JKE2

Additional TREM-1 Products

Product Documents for TREM1 Antibody (L5-B8.2A12.3A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TREM1 Antibody (L5-B8.2A12.3A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.