TREX1 Antibody (41M5F2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22394
![Western Blot: TREX1 Antibody (41M5F2) [NBP2-22394]](https://resources.bio-techne.com/images/products/TREX1-Antibody-41M5F2-Western-Blot-NBP2-22394-img0003.jpg "Western Blot: TREX1 Antibody (41M5F2) [NBP2-22394]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 41M5F2
Concentration
1.0 mg/ml
Product Summary for TREX1 Antibody (41M5F2)
Immunogen
Within the range of amino acids 211-350 of human TREX1 were used as immunogen for the antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for TREX1 Antibody (41M5F2)
Western Blot: TREX1 Antibody (41M5F2) [NBP2-22394]
Western Blot: TREX1 Antibody (41M5F2) [NBP2-22394] - Analysis of A) TREX1 partial recombinant protein (0.2 ug/ml) and B) human heart tissue (2 ug/ml) using TREX1 antibody.![Immunohistochemistry-Paraffin: TREX1 Antibody (41M5F2) [NBP2-22394]](https://resources.bio-techne.com/images/products/TREX1-Antibody-41M5F2-Immunohistochemistry-Paraffin-NBP2-22394-img0004.jpg "Immunohistochemistry-Paraffin: TREX1 Antibody (41M5F2) [NBP2-22394]")
Immunohistochemistry-Paraffin: TREX1 Antibody (41M5F2) [NBP2-22394]
Immunohistochemistry-Paraffin: TREX1 Antibody (41M5F2) [NBP2-22394] - Human spleen stained with TREX1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.![Flow Cytometry: TREX1 Antibody (41M5F2) [NBP2-22394]](https://resources.bio-techne.com/images/products/TREX1-Antibody-41M5F2-Flow-Cytometry-NBP2-22394-img0005.jpg "Flow Cytometry: TREX1 Antibody (41M5F2) [NBP2-22394]")
Flow Cytometry: TREX1 Antibody (41M5F2) [NBP2-22394]
Flow Cytometry: TREX1 Antibody (41M5F2) [NBP2-22394] - An intracellular stain was performed on HeLa cells with TREX1 [41M5F2] Antibody NBP2-22394AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.Applications for TREX1 Antibody (41M5F2)
Application
Recommended Usage
Flow Cytometry
2-5 ug/million cells/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
2-5 ug/ml
Application Notes
There are numerous isoforms of TREX1. Researchers are encouraged to use an online tool like NCBI's BLAST to determine if the antibody is suitable for use in their testing. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TREX1
Alternate Names
3'-5' exonuclease TREX1, AGS1, Aicardi-Goutieres syndrome 1, CRV, DKFZp434J0310, DNase III, DRN3, EC 3.1.11.2, HERNS, three prime repair exonuclease 1,3' repair exonuclease 1
Gene Symbol
TREX1
UniProt
Additional TREX1 Products
Product Documents for TREX1 Antibody (41M5F2)
Product Specific Notices for TREX1 Antibody (41M5F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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