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TRIB2 Antibody (OTI8D11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48030

Novus Biologicals, part of Bio-Techne
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NBP1-48030

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI8D11

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human TRIB2 (NP_067675) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

38.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIB2 Antibody (OTI8D11)

Western Blot: TRIB2 Antibody (OTI8D11) [NBP1-48030]

Western Blot: TRIB2 Antibody (OTI8D11) [NBP1-48030]

Western Blot: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TRIB2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: TRIB2 Antibody (OTI8D11) [NBP1-48030]

Immunocytochemistry/ Immunofluorescence: TRIB2 Antibody (OTI8D11) [NBP1-48030]

Immunocytochemistry/Immunofluorescence: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of COS7 cells using anti-TRIB2 mouse monoclonal antibody .
Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030]

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030]

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of paraffin-embedded Human endometrium tissue using anti-TRIB2 mouse monoclonal antibody.

Applications for TRIB2 Antibody (OTI8D11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIB2

This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene.

Alternate Names

C5FW, FLJ57420, GS3955, TRB-2, TRB2tribbles homolog 2, tribbles homolog 2 (Drosophila)

Entrez Gene IDs

28951 (Human)

Gene Symbol

TRIB2

Additional TRIB2 Products

Product Documents for TRIB2 Antibody (OTI8D11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRIB2 Antibody (OTI8D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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