TRIB3 Antibody (PSH02-13)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33050
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH02-13
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human TRIB3 aa 1-358 / 358. (Uniprot: Q96RU7)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRIB3 Antibody (PSH02-13)
Western Blot: TRIB3 Antibody (PSH02-13) [NBP3-33050]
Western Blot: TRIB3 Antibody (PSH02-13) [NBP3-33050] - Western blot analysis of TRIB3 on different lysates with Rabbit anti-TRIB3 antibody (NBP3-33050) at 1/1,000 dilution.Lane 1: HepG2 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: SK-Br-3 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: K-562 cell lysate
Lane 6: 293T cell lysate
Lane 7: HT-29 cell lysate
Lane 8: HUVEC cell lysate
Lysates/proteins at 40 ug/Lane.
Predicted band size: 40 kDa
Observed band size: 42 kDa
Exposure time: 2 minutes 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for TRIB3 Antibody (PSH02-13)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRIB3
Alternate Names
C20orf97, dJ1103G7.3, neuronal cell death inducible putative kinase, Neuronal cell death-inducible putative kinase, NIPK, p65-interacting inhibitor of NF-kappaB, p65-interacting inhibitor of NF-kappa-B, SINK, SKIP3, TRB-3, TRB3chromosome 20 open reading frame 97, tribbles homolog 3, tribbles homolog 3 (Drosophila)
Gene Symbol
TRIB3
Additional TRIB3 Products
Product Documents for TRIB3 Antibody (PSH02-13)
Product Specific Notices for TRIB3 Antibody (PSH02-13)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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