TRIM21/SSA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33548

Novus Biologicals, part of Bio-Techne
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NBP1-33548
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Co-IP, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRIM21 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM21/SSA1. The exact sequence is proprietary.

Reactivity Notes

Cat (86%), Pig (81%), Bovine (82%). Use in Mouse reported in scientific literature (PMID:32029896).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM21/SSA1 Antibody

Western Blot: TRIM21/SSA1 Antibody [NBP1-33548]

Western Blot: TRIM21/SSA1 Antibody [NBP1-33548]

Western Blot: TRIM21/SSA1 Antibody [NBP1-33548] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SSA1 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-33548]

Immunocytochemistry/ Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-33548]

Immunocytochemistry/Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-33548] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-33548]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-33548]

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-33548] - DLD-1 xenograft , using SSA1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
View 1 more images

Applications for TRIM21/SSA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Knockdown Validated

Reported in scientific literature (PMID:32029896)

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRIM21

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Long Name

Tripartite Motif-containing Protein 21

Alternate Names

RNF81, Ro52, SSA1

Entrez Gene IDs

6737 (Human)

Gene Symbol

TRIM21

UniProt

P19474 (Human)

Additional TRIM21 Products

Product Documents for TRIM21/SSA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRIM21/SSA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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