TRIM25 Antibody (5F12)

Novus Biologicals, part of Bio-Techne | Catalog # H00007706-M02

Novus Biologicals, part of Bio-Techne
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H00007706-M02
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRIM25 Antibodies »

Product Specifications

Immunogen

TRIM25 (NP_005073, 211 a.a. ~ 310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEK

Specificity

TRIM25 - tripartite motif-containing 25 (5F12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TRIM25 Antibody (5F12)

Western Blot: TRIM25 Antibody (5F12) [H00007706-M02]

Western Blot: TRIM25 Antibody (5F12) [H00007706-M02]

Western Blot: TRIM25 Antibody (5F12) [H00007706-M02] - TRIM25 monoclonal antibody (M02), clone 5F12 Analysis of TRIM25 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody (5F12) [H00007706-M02]

Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody (5F12) [H00007706-M02]

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5F12) [H00007706-M02] - Analysis of monoclonal antibody to TRIM25 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: TRIM25 Antibody (5F12) [H00007706-M02]

ELISA: TRIM25 Antibody (5F12) [H00007706-M02]

ELISA: TRIM25 Antibody (5F12) [H00007706-M02] - Detection limit for recombinant GST tagged TRIM25 is 0.3 ng/ml as a capture antibody.

Applications for TRIM25 Antibody (5F12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRIM25

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]

Long Name

Tripartite Motif-containing Protein 25

Alternate Names

EFP, RNF147, ZNF147

Entrez Gene IDs

7706 (Human)

Gene Symbol

TRIM25

UniProt

NP_005073 (Human)

Additional TRIM25 Products

Product Documents for TRIM25 Antibody (5F12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRIM25 Antibody (5F12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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