TRIM25 Antibody (SR2171)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21633

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21633-100ul
NBP3-21633-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2171

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRIM25 Antibodies »

Product Summary for TRIM25 Antibody (SR2171)

Immunogen

A synthesized peptide derived from human TRIM25 (Uniprot #: Q14258)

Specificity

Detects endogenous levels of total TRIM25

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM25 Antibody (SR2171)

Western Blot: TRIM25 Antibody (SR2171) [NBP3-21633] -

Western Blot: TRIM25 Antibody (SR2171) [NBP3-21633] - Western blot analysis using NBP3-21633 in HeLa cell lysate.

Applications for TRIM25 Antibody (SR2171)

Application
Recommended Usage

Flow Cytometry

1:60

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM25

TRIM25 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Long Name

Tripartite Motif-containing Protein 25

Alternate Names

EFP, RNF147, ZNF147

Gene Symbol

TRIM25

Additional TRIM25 Products

Product Documents for TRIM25 Antibody (SR2171)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRIM25 Antibody (SR2171)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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