TRIM25 Antibody

Western Blot: TRIM25 Antibody [NBP2-20710]

Western Blot: TRIM25 Antibody [NBP2-20710] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody diluted at 1:500.

Western Blot: TRIM25 Antibody [NBP2-20710]

Western Blot: TRIM25 Antibody [NBP2-20710] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: TRIM25 Antibody [NBP2-20710]

Immunoprecipitation: TRIM25 Antibody [NBP2-20710] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TRIM25 protein by 2 ug of TRIM25 antibody 7.5% SDS-PAGE The immunoprecipitated TRIM25 protein was detected by TRIM25 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Knockdown Validated: TRIM25 Antibody [NBP2-20710]

Knockdown Validated: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis of UPR signaling, including phospho-JNK/JNK and phospho-eIF2alpha/eIF2alpha in HCT116 cells after stable knockdown of TRIM25 in HCT116 cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.

Immunohistochemistry: TRIM25 Antibody [NBP2-20710] -

Immunohistochemistry: TRIM25 Antibody [NBP2-20710] - Knockdown of TRIM25 in HCC suppresses tumor growth in nude mice.a–c Huh7 cells stably expressing the indicated shRNAs (shNC, shTRIM25-1 & shTRIM25-2) were subcutaneously injected in nude mice respectively. Shown are average tumor volumes over time (n = 8) (a) & representative image (b) & weights (c) of tumors at day 25. d Kaplan–Meier analysis of overall survival percentage in nude mice inoculated with Huh7-shNC or Huh7-shTRIM25. The statistical significance was assessed using two-sided log-rank test according to mice with depletion of control or TRIM25. e, f Representative images (e) of IHC staining & the relative IHC scores (n = 8) (f) of TRIM25, Ki67, Keap1 & Nrf2 in HCC tissues of mice inoculated with Huh7-shNC or Huh7-shTRIM25-1&-2. Scale bar, 50 μm. g, h Representative IHC staining images (g) & statistical data (h) of TRIM25, Keap1 & Nrf2 expression in HCC tissues (TRIM25 low expression group, n = 45 & TRIM25 high expression group, n = 45). Scale bar, 50 μm. For a, c, f, & h, data represent the mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-tests. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31953436), licensed under a CC-BY license. Not internally tested by Novus Biologicals.