Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TRIM27 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TRIM27 (NP_006501) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

58.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM27 Antibody (OTI4D4) - Azide and BSA Free

Western Blot: TRIM27 Antibody (OTI4D4)Azide and BSA Free [NBP2-74609]

Western Blot: TRIM27 Antibody (OTI4D4)Azide and BSA Free [NBP2-74609]

Western Blot: TRIM27 Antibody (OTI4D4) - Azide and BSA Free [NBP2-74609] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM27.
Immunohistochemistry: TRIM27 Antibody (OTI4D4) - Azide and BSA Free [NBP2-74609]

Immunohistochemistry: TRIM27 Antibody (OTI4D4) - Azide and BSA Free [NBP2-74609]

Immunohistochemistry: TRIM27 Antibody (OTI4D4) - Azide and BSA Free [NBP2-74609] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: TRIM27 Antibody (OTI4D4) - Azide and BSA Free [NBP2-74609]

Immunohistochemistry: TRIM27 Antibody (OTI4D4) - Azide and BSA Free [NBP2-74609]

Immunohistochemistry: TRIM27 Antibody (OTI4D4) - Azide and BSA Free [NBP2-74609] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for TRIM27 Antibody (OTI4D4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM27

The TRIM27 gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the

Alternate Names

Ret finger proteinRING finger protein 76, RFPtripartite motif protein TRIM27, RNF76RFP transforming protein, tripartite motif containing 27, tripartite motif-containing 27, Tripartite motif-containing protein 27, zinc finger protein RFP

Gene Symbol

TRIM27

Additional TRIM27 Products

Product Documents for TRIM27 Antibody (OTI4D4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRIM27 Antibody (OTI4D4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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