TRIM27 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54993

Novus Biologicals, part of Bio-Techne
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NBP2-54993
NBP2-54993-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRIM27 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM27 Antibody

Western Blot: TRIM27 Antibody [NBP2-54993]

Western Blot: TRIM27 Antibody [NBP2-54993]

Western Blot: TRIM27 Antibody [NBP2-54993] - Western blot analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: TRIM27 Antibody [NBP2-54993]

Immunocytochemistry/ Immunofluorescence: TRIM27 Antibody [NBP2-54993]

Immunocytochemistry/Immunofluorescence: TRIM27 Antibody [NBP2-54993] - Staining of human cell line A-431 shows localization to nucleoli fibrillar center.
Western Blot: TRIM27 Antibody [NBP2-54993]

Western Blot: TRIM27 Antibody [NBP2-54993]

Western Blot: TRIM27 Antibody [NBP2-54993] - Analysis in human cell lines U2OS and RT-4. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for TRIM27 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM27

The TRIM27 gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the

Alternate Names

Ret finger proteinRING finger protein 76, RFPtripartite motif protein TRIM27, RNF76RFP transforming protein, tripartite motif containing 27, tripartite motif-containing 27, Tripartite motif-containing protein 27, zinc finger protein RFP

Gene Symbol

TRIM27

Additional TRIM27 Products

Product Documents for TRIM27 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRIM27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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