TRIM32 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13484

Novus Biologicals, part of Bio-Techne
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NBP2-13484
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRIM32 Antibodies »

Product Summary for TRIM32 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: NLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM32 Antibody

Western Blot: TRIM32 Antibody [NBP2-13484]

Western Blot: TRIM32 Antibody [NBP2-13484]

Western Blot: TRIM32 Antibody [NBP2-13484] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody [NBP2-13484]

Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody [NBP2-13484]

Immunocytochemistry/Immunofluorescence: TRIM32 Antibody [NBP2-13484] - Immunofluorescent staining of human cell line A-431 shows localization to intermediate filaments.
Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP2-13484]

Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP2-13484]

Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP2-13484] - Staining of human gallbladder shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for TRIM32 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM32

TRIM32 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Long Name

Tripartite Motif-containing Protein 32

Alternate Names

BBS11, HT2A, LGMD2H, TATIP

Gene Symbol

TRIM32

Additional TRIM32 Products

Product Documents for TRIM32 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRIM32 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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