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TRIM35 Antibody (4F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00023087-M02

Novus Biologicals, part of Bio-Techne
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H00023087-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

TRIM35 (NP_055881, 394 a.a. ~ 493 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SGFWYVCRTQGVEGDHCVTSDPATSPLVLAIPRRLRVELECEEGELSFYDAERHCHLYTFHARFGEVRPYFYLGGARGAGPPEPLRICPLHISVKEELDG

Reactivity Notes

Human. Other species not tested.

Specificity

TRIM35 - tripartite motif-containing 35

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TRIM35 Antibody (4F7)

Western Blot: TRIM35 Antibody (4F7) [H00023087-M02]

Western Blot: TRIM35 Antibody (4F7) [H00023087-M02]

Western Blot: TRIM35 Antibody (4F7) [H00023087-M02] - TRIM35 expression in K-562 ( Cat # L009V1 ).
Immunohistochemistry-Paraffin: TRIM35 Antibody (4F7) [H00023087-M02]

Immunohistochemistry-Paraffin: TRIM35 Antibody (4F7) [H00023087-M02]

Immunohistochemistry-Paraffin: TRIM35 Antibody (4F7) [H00023087-M02] - Analysis of monoclonal antibody to TRIM35 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
Sandwich ELISA: TRIM35 Antibody (4F7) [H00023087-M02] - Detection limit for recombinant GST tagged TRIM35 is approximately 0.03ng/ml as a capture antibody.

Applications for TRIM35 Antibody (4F7)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against cell lysate for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRIM35

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified.

Alternate Names

HLS5Hemopoietic lineage switch protein 5, KIAA1098MGC17233, MAIR, tripartite motif containing 35, tripartite motif-containing 35, tripartite motif-containing protein 35

Entrez Gene IDs

23087 (Human)

Gene Symbol

TRIM35

Additional TRIM35 Products

Product Documents for TRIM35 Antibody (4F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRIM35 Antibody (4F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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