TRIM37 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98831

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRIM37 Antibodies »

Product Summary for TRIM37 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TRIM37.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TRIM37 Antibody

Western Blot: TRIM37 Antibody [NBP2-98831]

Western Blot: TRIM37 Antibody [NBP2-98831]

Western Blot: TRIM37 Antibody [NBP2-98831] - Anti-TRIM37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 108 kDa. Observed band size: 128 kDa
Immunocytochemistry/ Immunofluorescence: TRIM37 Antibody [NBP2-98831]

Immunocytochemistry/ Immunofluorescence: TRIM37 Antibody [NBP2-98831]

Immunocytochemistry/Immunofluorescence: TRIM37 Antibody [NBP2-98831] - Immunofluorescence staining of TRIM37 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti- TRIM37 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.

Applications for TRIM37 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM37

TRIM37 encodes a member of the tripartite motif (TRIM) family, whose members are involved in diverse cellular functions such as developmental patterning and oncogenesis. The TRIM motif includes zinc-binding domains, a RING finger region, a B-box motif and a coiled-coil domain. The RING finger and B-box domains chelate zinc and might be involved in protein-protein and/or protein-nucleic acid interactions. The gene mutations are associated with mulibrey (muscle-liver-brain-eye) nanism, an autosomal recessive disorder that involves several tissues of mesodermal origin. Alternatively spliced transcript variants encoding the same protein have been identified.

Alternate Names

EC 6.3.2.-, KIAA0898Tripartite motif-containing protein 37, Mulibrey nanism protein, MULRING-B-box-coiled-coil protein, POB1E3 ubiquitin-protein ligase TRIM37, TEF3, tripartite motif containing 37, tripartite motif-containing 37

Gene Symbol

TRIM37

Additional TRIM37 Products

Product Documents for TRIM37 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRIM37 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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